A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in <i>Trichoderma reesei</i>

Abstract

<jats:p> In this work, we used a systems biology approach to map new regulatory interactions in <jats:named-content content-type="genus-species">Trichoderma reesei</jats:named-content> controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. <jats:italic>In vivo</jats:italic> experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms. </jats:p>